Paolo Odogwu and Harry Randall receive their first call-ups to the senior team, both having played for England at U20 level.
Owen Farrell will captain England for the tournament and there is a return from injury for Courtney Lawes.
Elsewhere, Elliot Daly and Anthony Watson could reach their 50-cap milestones during the championship and Beno Obano may be in line to get his first cap.
England will retain a 28-player squad throughout the tournament, following an agreement with Premiership Rugby, The RPA and The RFU in recognition of the challenges presented by COVID-19.
The squad will meet up at St. George’s Park, England football’s training base, on Wednesday 27 January. They will then move to their base, The Lensbury in Teddington, ahead of their first game against Scotland at Twickenham Stadium on Saturday 6 February.
Jones said: “This has been a really tough squad to pick and I know there are a lot of disappointed players who haven’t been selected. We’re really excited by the players we have got, it’s a very vibrant squad and are looking forward to getting started next week.
“We’re very grateful to the Premiership Rugby, the clubs and RPA for allowing us to have 28 players throughout the tournament, we’re delighted with the number and it’s testament to the growing relationships between all parties.
“Ultimately our goal is to win the Six Nations and to do that we’ll need to work hard and prepare well each day and take it as it comes.”
Jones has also named a 12-player shadow squad, who will be part of the same testing protocols as the tournament squad. Mako Vunipola will join with the squad to undertake rehab during the tournament.
Following their opening match, the Calcutta Cup, England will then host Italy on Saturday 13 February before playing Wales away on Saturday 27 February ).
Jones’ side will return to Twickenham to face France on Saturday 13 March before travelling to Dublin for their final game, against Ireland, on Saturday 20 March.
Elliot Daly (Saracens, 47 caps)
Owen Farrell (Saracens, 88 caps)
George Ford (Leicester Tigers, 72 caps)
Ollie Lawrence (Worcester Warriors, 3 caps)
Max Malins (Bristol Bears, 3 caps)
Jonny May (Gloucester Rugby, 61 caps)
Paolo Odogwu (Wasps, uncapped)
Harry Randall (Bristol Bears, uncapped)
Dan Robson (Wasps, 7 caps)
Henry Slade (Exeter Chiefs, 34 caps)
Anthony Watson (Bath Rugby, 46 caps)
Ben Youngs (Leicester Tigers, 104 caps)
Luke Cowan-Dickie (Exeter Chiefs, 26 caps)
Tom Curry (Sale Sharks, 28 caps)
Ben Earl (Bristol Bears, 8 caps)
Ellis Genge (Leicester Tigers, 23 caps)
Jamie George (Saracens, 54 caps)
Jonny Hill (Exeter Chiefs, 3 caps)
Maro Itoje (Saracens, 43 caps)
Joe Launchbury (Wasps, 69 caps)
Courtney Lawes (Northampton Saints, 85 caps)
Joe Marler (Harlequins, 72 caps)
Beno Obano (Bath Rugby, uncapped)
Will Stuart (Bath Rugby, 8 caps)
Sam Underhill (Bath Rugby, 22 caps)
Billy Vunipola (Saracens, 56 caps)
Harry Williams (Exeter Chiefs, 18 caps)
Mark Wilson (Newcastle Falcons, 19 caps)
Charlie Atkinson (Wasps, uncapped)
Ali Crossdale (Saracens, uncapped)
Tom Dunn (Bath Rugby, 3 caps)
Charlie Ewels (Bath Rugby, 17 caps)
George Furbank (Northampton Saints, 3 caps)
Joe Heyes (Leicester Tigers, uncapped)
Jonathan Joseph (Bath Rugby, 54 caps)
Joe Marchant (Harlequins, 4 caps)
George Martin (Leicester Tigers, uncapped)
Alex Mitchell (Northampton Saints, uncapped)
Jacob Umaga (Wasps, uncapped)
Jack Willis (Wasps, 2 caps)